Host cell-dependent regulation of growth transformation-associated Epstein-Barr virus antigens in somatic cell hybrids

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Amounts of Epstein-Barr virus DNA in somatic cell hybrids between burkitt lymphoma-derived cell lines.

The amounts of Epstein-Barr virus DNA in somatic cell hybrids between human lymphoid cell lines were found to be higher than in the parental cell lines, although the hybrids were not induced to spontaneous virus production.

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When Epstein-Barr virus (EBV) negative cells (Raji) were treated with iododeoxyuridine, only the early antigen (EA) component was induced. There was no significant increase in EBV DNA and no virus particles were observed. Somatic-cell hybrids were prepared from the fusion of Raji and D98 cells (D98/Raji). When these cells were treated with iododeoxyuridine, early antigen EBV DNA, and virus part...

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Epstein-Barr Virus-Associated Gastric Carcinoma: Use of Host Cell Machineries and Somatic Gene Mutations.

Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) is a distinct subtype of gastric carcinoma, consisting of clonal growth of EBV-infected epithelial cells. Its unique characteristics have been demonstrated by epidemiological, clinical and pathological studies using in situ hybridization for EBV-encoded small RNAs. An oncogenic process for EBVaGC has also been revealed. EBV uses var...

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THE IN VITRO GROWTH PROPERTIES OF CELL LINES FROM EPSTEIN-BARR VIRUS-INDUCED TAMARIN TUMORS AND TAMARIN B CELLS TR ANSFORMED BY EPSTEIN BARR VIRUS

EBV-carrying human cell lines, depending on whether the cells are derived from Burkitt's lymphoma (BL) tumor biopsies or transformed by EBV in vitro, have different growth properties in vitro. In contrast, there are no clear differences between tamarin tumor lines and tamarin LCLs in vitro. Both types of tamarin cell lines could grow in agarose and formed colonies unlike human LCLs, althoug...

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Rescue of Epstein-Barr virus from somatic cell hybrids of Burkitt lymphoblastoid cells.

A Burkitt lymphoblastoid cell line, P3J-HR-1, was fused and hybridized to a human sternal marrow cell line. The somatic cell hybrids were negative when examined for Epstein-Barr virus (EBV) markers. When the hybrid cells were exposed to 5-iododeoxyuridine, both EBV-specific antigens and virus particles were induced as determined by the immunofluorescence test and by electron microscopy. The dat...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1989

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.63.6.2768-2772.1989